Preparation, release and antitumor properties of sodium alginate/layered double hydroxides-5- fluorouracil delivery system

doi:10.3969/j.issn.2095-4344.0943

Abstract

Abstract:

BACKGROUND: In clinical practice, 5-fluorouracil (5-Fu) has disadvantages such as high toxicity and short metabolism time.

OBJECTIVE: To prepare the sodium alginate (SA)/layered double hydroxides (LDH)-5-Fu delivery system and to explore its drug loading, micromorphology, in vitro release and inhibitory effect on hepatoma cells.

METHODS: LDH-5-Fu solution and SA solution were mixed at a ratio of 3:5, 4:5 and 5:5 to prepare SA/LDH-5-Fu beads using ionic gel method, respectively. The drug loading capacity of these beads was then tested. SA/LDH-5-Fu beads were placed in PBS of pH=2.1, 4.6, and 7.4, and the release rate of 5-Fu was measured periodically. SMMC-7 721 hepatoma cells were cultured with the SA at different concentrations (5, 10, 20, 40 mg/L), LDH-5-Fu beads, and SA/LDH-5-Fu bead suspension for 24 hours, and then the cell inhibitory rate was detected.

RESULTS AND CONCLUSION: The drug loading rate of SA/LDH-5-Fu at 3:5, 4:5, and 5:5 was (3.09±0.08)%, (4.55±0.10)%, and (5.47±0.14)%, respectively. The release of 5-Fu from the SA/LDH-5-Fu beads at pH=2.1 and 4.6 was faster than that at pH=7.4. Moreover, the release of 5-Fu was gradually fastened with the increasing input amount of 5-Fu. SA showed little inhibition effect on SMMC-7721 cells, while the inhibitory rate of LDH-5-Fu or SA/LDH-5-Fu to SMMC-7721 in vitro was gradually increased, and the inhibitory rate of SA/LDH-5-Fu was significantly higher than that of LDH-5-Fu. These findings reveal that SA/LDH-5-Fu is a potential pH sensitive anti-tumor drug release system.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Fluorouracil, Liver Neoplasms, Tissue Engineering

CLC Number:

R318

Han Xiao-jing, Yang Hong, Sun Xiao-yu, Wang Hui, Wang Qing-feng. Preparation, release and antitumor properties of sodium alginate/layered double hydroxides-5- fluorouracil delivery system[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(26): 4168-4173.

Figures/Tables 5

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